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What is a AFB STAIN SPUTUM?

AFB Stain Sputum is a laboratory test that examines a sputum sample under a microscope for acid-fast bacilli (AFB), bacteria that retain special stains and include Mycobacterium tuberculosis, the organism responsible for tuberculosis (TB). The test is needed when pulmonary TB or another mycobacterial infection is suspected, particularly in individuals with persistent cough, fever, night sweats, weight loss, or abnormal chest imaging.

Additional Information

Also known as/ Other names: Sputum AFB Smear Test, AFB Smear for Sputum, Sputum for Acid Fast Bacilli, Ziehl–Neelsen Stain – Sputum
Parameters: 1
Recommended for: M/F/Others
Sample Type: Sputum
Patient Preparation: Early morning sample is preferred.
Reports: Next Day

FAQs

What does AFB stand for?

AFB stands for acid-fast bacilli. These bacteria retain certain stains even after treatment with an acid-based decolorizing solution, allowing them to be identified microscopically.

Does a positive AFB stain mean I have tuberculosis?

Not necessarily. A positive result indicates that acid-fast organisms were detected, but some non-tuberculous mycobacteria can also produce a positive result. Additional testing may be needed.

Does a negative AFB stain rule out tuberculosis?

No. A negative smear does not completely exclude TB because the number of organisms in sputum may be too low for microscopic detection. Molecular or culture testing may be required.

Why are multiple sputum samples sometimes requested?

Multiple specimens can improve the likelihood of detecting mycobacteria because the number of organisms may vary between samples. Your healthcare provider or TB program will determine the appropriate sampling schedule.

What is the difference between AFB smear and TB PCR?

AFB smear uses microscopy to look for acid-fast organisms, while PCR detects genetic material from specific organisms. Molecular testing can provide more specific information about M. tuberculosis.

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